Regeneration Ability and Agrobacterium-mediated Transformation of Different Cultivars in Brassica oleracea L. and B. rapa L. (syn. B. campestris L.).
نویسندگان
چکیده
منابع مشابه
Regeneration Potential of Five Cultivars of Canola (Brassica Napus L.) And Agrobacterium-mediated Transformation with the Salt Tolerance Gene mtlD
In order to improve the salt tolerance of some canola genotypes to be suitable for cultivation in Kingdom of Saudi Arabia (KSA), the salt tolerance gene (mt1D) encoding for mannitol-1-phosphate dehydrogenase was introduced to the canola plants using the agrobacterium-mediated transformation. To achieve that, five canola genotypes were selected as a relatively salt tolerance among 15 canola geno...
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A method for transformation of B.napus is presented in this study. The protocol is based on infection of cotyledonary petioles of 5-day-old and hypocotyl segments of 14-day-old seedlings with an Agrobacterium tumefaciens strain LBA 4404 carrying a disarmed binary vector pBI121. The T-DNA region of this binary vector contains the neomycin phosphotransferase II (NPTII) and glucuronidase (GUS) gen...
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Genetic transformation studies were carried out to standardize a protocol for Agrobacterium -mediated genetic transformation of three economically important strawberry (Fragaria x ananassa Duch) cultivars Kurdistan’, Camarosa’, and ‘Paros’. Shoot regeneration frequency 72, 65 and 30% was obtained on MS (1) basal medium supplemented with 2% glucose and 4 mg/l TDZ for Camarosa, Kurdistan and Paro...
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Agrobacterium tumefaciens mediated transformation may offer a better alternative than the biolistic gun for genetic transformation of maize plants. This gene delivery system results in a greater proportion of stable, low-copy number transgenic events than does the biolistic gun, and is highly efficient. In the present work, we studied maize transformation using A. tumefaciens by identifying som...
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The herbicide cilluron containing cyhalofop-butyl (10%) is an active ingredient. Cyhalofop-butyl has low oral, dermal and inhalation toxicity. In the present study the antigenotoxic effects of Brassica oleracea L. var. italica was evaluated using Allium cepa root chromosomal aberration assay against ciluron. Three types of treatments were given to roots in pre-treatment roots were first treated...
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ژورنال
عنوان ژورنال: Engei Gakkai zasshi
سال: 2001
ISSN: 1880-358X,0013-7626
DOI: 10.2503/jjshs.70.682